diff --git a/cli/lsp/config.rs b/cli/lsp/config.rs
index 2c8d007035..8b31cc35fc 100644
--- a/cli/lsp/config.rs
+++ b/cli/lsp/config.rs
@@ -239,6 +239,10 @@ pub struct SpecifierSettings {
   /// A flag that indicates if Deno is enabled for this specifier or not.
   pub enable: Option<bool>,
   /// A list of paths, using the workspace folder as a base that should be Deno
+  /// disabled.
+  #[serde(default)]
+  pub disable_paths: Vec<String>,
+  /// A list of paths, using the workspace folder as a base that should be Deno
   /// enabled.
   pub enable_paths: Option<Vec<String>>,
   /// Code lens specific settings for the resource.
@@ -285,6 +289,11 @@ pub struct WorkspaceSettings {
   /// A flag that indicates if Deno is enabled for the workspace.
   pub enable: Option<bool>,
 
+  /// A list of paths, using the root_uri as a base that should be Deno
+  /// disabled.
+  #[serde(default)]
+  pub disable_paths: Vec<String>,
+
   /// A list of paths, using the root_uri as a base that should be Deno enabled.
   pub enable_paths: Option<Vec<String>>,
 
@@ -353,6 +362,7 @@ impl Default for WorkspaceSettings {
   fn default() -> Self {
     WorkspaceSettings {
       enable: None,
+      disable_paths: vec![],
       enable_paths: None,
       cache: None,
       certificate_stores: None,
@@ -643,34 +653,96 @@ impl Config {
   pub fn enabled_urls(&self) -> Vec<Url> {
     let mut urls = vec![];
     for (workspace_uri, _) in &self.workspace_folders {
+      let Ok(workspace_path) = specifier_to_file_path(workspace_uri) else {
+        lsp_log!("Unable to convert uri \"{}\" to path.", workspace_uri);
+        continue;
+      };
       let specifier_settings = self.settings.specifiers.get(workspace_uri);
       let enable = specifier_settings
         .and_then(|s| s.enable)
         .or(self.settings.workspace.enable)
         .unwrap_or(self.has_config_file());
+      let disable_paths = specifier_settings
+        .map(|s| &s.disable_paths)
+        .unwrap_or(&self.settings.workspace.disable_paths);
+      let resolved_disable_paths = disable_paths
+        .iter()
+        .map(|p| workspace_path.join(p))
+        .collect::<Vec<_>>();
       let enable_paths = specifier_settings
         .and_then(|s| s.enable_paths.as_ref())
         .or(self.settings.workspace.enable_paths.as_ref());
       if let Some(enable_paths) = enable_paths {
-        let Ok(scope_path) = specifier_to_file_path(workspace_uri) else {
-          lsp_log!("Unable to convert uri \"{}\" to path.", workspace_uri);
-          return vec![];
-        };
         for path in enable_paths {
-          let path = scope_path.join(path);
+          let path = workspace_path.join(path);
           let Ok(path_uri) = specifier_from_file_path(&path) else {
             lsp_log!("Unable to convert path \"{}\" to uri.", path.display());
             continue;
           };
-          urls.push(path_uri);
+          if !resolved_disable_paths.iter().any(|p| path.starts_with(p)) {
+            urls.push(path_uri);
+          }
         }
-      } else if enable {
+      } else if enable
+        && !resolved_disable_paths
+          .iter()
+          .any(|p| workspace_path.starts_with(p))
+      {
         urls.push(workspace_uri.clone());
       }
     }
 
     // sort for determinism
     urls.sort();
+    urls.dedup();
+    urls
+  }
+
+  pub fn disabled_urls(&self) -> Vec<Url> {
+    let root_enable = self
+      .settings
+      .workspace
+      .enable
+      .unwrap_or(self.has_config_file());
+    let mut urls = vec![];
+    if let Some(cf) = self.maybe_config_file() {
+      if let Some(files) = cf.to_files_config().ok().flatten() {
+        for path in files.exclude {
+          let Ok(path_uri) = specifier_from_file_path(&path) else {
+            lsp_log!("Unable to convert path \"{}\" to uri.", path.display());
+            continue;
+          };
+          urls.push(path_uri);
+        }
+      }
+    }
+    for (workspace_uri, _) in &self.workspace_folders {
+      let Ok(workspace_path) = specifier_to_file_path(workspace_uri) else {
+        lsp_log!("Unable to convert uri \"{}\" to path.", workspace_uri);
+        continue;
+      };
+      let specifier_settings = self.settings.specifiers.get(workspace_uri);
+      let enable = specifier_settings
+        .and_then(|s| s.enable)
+        .unwrap_or(root_enable);
+      if enable {
+        let disable_paths = specifier_settings
+          .map(|s| &s.disable_paths)
+          .unwrap_or(&self.settings.workspace.disable_paths);
+        for path in disable_paths {
+          let path = workspace_path.join(path);
+          let Ok(path_uri) = specifier_from_file_path(&path) else {
+            lsp_log!("Unable to convert path \"{}\" to uri.", path.display());
+            continue;
+          };
+          urls.push(path_uri);
+        }
+      } else {
+        urls.push(workspace_uri.clone());
+      }
+    }
+    urls.sort();
+    urls.dedup();
     urls
   }
 
@@ -778,9 +850,9 @@ fn specifier_enabled(
 
   let root_enable = settings.workspace.enable.unwrap_or(config_file.is_some());
   if let Some(settings) = settings.specifiers.get(specifier) {
-    // TODO(nayeemrmn): We don't know from where to resolve `enable_paths` in
-    // this case. If it's detected, instead defer to workspace scopes.
-    if settings.enable_paths.is_none() {
+    // TODO(nayeemrmn): We don't know from where to resolve path lists in this
+    // case. If they're detected, instead defer to workspace scopes.
+    if settings.enable_paths.is_none() && settings.disable_paths.is_empty() {
       return settings.enable.unwrap_or(root_enable);
     }
   }
@@ -795,13 +867,22 @@ fn specifier_enabled(
     };
     if path.starts_with(&workspace_path) {
       let specifier_settings = settings.specifiers.get(workspace_uri);
+      let disable_paths = specifier_settings
+        .map(|s| &s.disable_paths)
+        .unwrap_or(&settings.workspace.disable_paths);
+      let resolved_disable_paths = disable_paths
+        .iter()
+        .map(|p| workspace_path.join(p))
+        .collect::<Vec<_>>();
       let enable_paths = specifier_settings
         .and_then(|s| s.enable_paths.as_ref())
         .or(settings.workspace.enable_paths.as_ref());
       if let Some(enable_paths) = enable_paths {
         for enable_path in enable_paths {
           let enable_path = workspace_path.join(enable_path);
-          if path.starts_with(&enable_path) {
+          if path.starts_with(&enable_path)
+            && !resolved_disable_paths.iter().any(|p| path.starts_with(p))
+          {
             return true;
           }
         }
@@ -809,7 +890,8 @@ fn specifier_enabled(
       } else {
         return specifier_settings
           .and_then(|s| s.enable)
-          .unwrap_or(root_enable);
+          .unwrap_or(root_enable)
+          && !resolved_disable_paths.iter().any(|p| path.starts_with(p));
       }
     }
   }
@@ -919,6 +1001,20 @@ mod tests {
     assert!(!config_snapshot.specifier_enabled(&specifier_b));
   }
 
+  #[test]
+  fn test_config_specifier_disabled_path() {
+    let root_uri = resolve_url("file:///root/").unwrap();
+    let mut config = Config::new_with_root(root_uri.clone());
+    config.settings.workspace.enable = Some(true);
+    config.settings.workspace.enable_paths =
+      Some(vec!["mod1.ts".to_string(), "mod2.ts".to_string()]);
+    config.settings.workspace.disable_paths = vec!["mod2.ts".to_string()];
+
+    assert!(config.specifier_enabled(&root_uri.join("mod1.ts").unwrap()));
+    assert!(!config.specifier_enabled(&root_uri.join("mod2.ts").unwrap()));
+    assert!(!config.specifier_enabled(&root_uri.join("mod3.ts").unwrap()));
+  }
+
   #[test]
   fn test_set_workspace_settings_defaults() {
     let mut config = Config::new();
@@ -929,6 +1025,7 @@ mod tests {
       config.workspace_settings().clone(),
       WorkspaceSettings {
         enable: None,
+        disable_paths: vec![],
         enable_paths: None,
         cache: None,
         certificate_stores: None,
@@ -1135,13 +1232,18 @@ mod tests {
     let mut config = Config::new_with_root(root_uri.clone());
     config.settings.workspace.enable = Some(true);
 
-    config.settings.workspace.enable_paths = Some(vec!["mod1.ts".to_string()]);
+    config.settings.workspace.enable_paths =
+      Some(vec!["mod1.ts".to_string(), "mod2.ts".to_string()]);
+    config.settings.workspace.disable_paths = vec!["mod2.ts".to_string()];
     assert!(
       config.specifier_enabled_for_test(&root_uri.join("mod1.ts").unwrap())
     );
     assert!(
       !config.specifier_enabled_for_test(&root_uri.join("mod2.ts").unwrap())
     );
+    assert!(
+      !config.specifier_enabled_for_test(&root_uri.join("mod3.ts").unwrap())
+    );
     config.settings.workspace.enable_paths = None;
 
     config.set_config_file(
diff --git a/cli/lsp/diagnostics.rs b/cli/lsp/diagnostics.rs
index 361dba1966..a04f490f70 100644
--- a/cli/lsp/diagnostics.rs
+++ b/cli/lsp/diagnostics.rs
@@ -1477,6 +1477,7 @@ let c: number = "a";
         specifier.clone(),
         SpecifierSettings {
           enable: Some(false),
+          disable_paths: vec![],
           enable_paths: None,
           code_lens: Default::default(),
         },
diff --git a/cli/lsp/documents.rs b/cli/lsp/documents.rs
index 388f9f2e56..e601147ffb 100644
--- a/cli/lsp/documents.rs
+++ b/cli/lsp/documents.rs
@@ -804,6 +804,7 @@ impl FileSystemDocuments {
 
 pub struct UpdateDocumentConfigOptions<'a> {
   pub enabled_urls: Vec<Url>,
+  pub disabled_urls: Vec<Url>,
   pub document_preload_limit: usize,
   pub maybe_import_map: Option<Arc<import_map::ImportMap>>,
   pub maybe_config_file: Option<&'a ConfigFile>,
@@ -1283,14 +1284,10 @@ impl Documents {
           .filter_map(|url| specifier_to_file_path(url).ok())
           .collect(),
         options
-          .maybe_config_file
-          .and_then(|cf| {
-            cf.to_files_config()
-              .ok()
-              .flatten()
-              .map(|files| files.exclude)
-          })
-          .unwrap_or_default(),
+          .disabled_urls
+          .iter()
+          .filter_map(|url| specifier_to_file_path(url).ok())
+          .collect(),
         options.document_preload_limit,
       );
       self.resolver_config_hash = new_resolver_config_hash;
@@ -2032,6 +2029,7 @@ console.log(b, "hello deno");
 
       documents.update_config(UpdateDocumentConfigOptions {
         enabled_urls: vec![],
+        disabled_urls: vec![],
         document_preload_limit: 1_000,
         maybe_import_map: Some(Arc::new(import_map)),
         maybe_config_file: None,
@@ -2073,6 +2071,7 @@ console.log(b, "hello deno");
 
       documents.update_config(UpdateDocumentConfigOptions {
         enabled_urls: vec![],
+        disabled_urls: vec![],
         document_preload_limit: 1_000,
         maybe_import_map: Some(Arc::new(import_map)),
         maybe_config_file: None,
diff --git a/cli/lsp/language_server.rs b/cli/lsp/language_server.rs
index 26d03b76d7..385de0ba1a 100644
--- a/cli/lsp/language_server.rs
+++ b/cli/lsp/language_server.rs
@@ -1354,6 +1354,7 @@ impl Inner {
   async fn refresh_documents_config(&mut self) {
     self.documents.update_config(UpdateDocumentConfigOptions {
       enabled_urls: self.config.enabled_urls(),
+      disabled_urls: self.config.disabled_urls(),
       document_preload_limit: self
         .config
         .workspace_settings()
diff --git a/cli/lsp/repl.rs b/cli/lsp/repl.rs
index c21e2e3fba..c09a142d68 100644
--- a/cli/lsp/repl.rs
+++ b/cli/lsp/repl.rs
@@ -285,6 +285,7 @@ fn get_cwd_uri() -> Result<ModuleSpecifier, AnyError> {
 pub fn get_repl_workspace_settings() -> WorkspaceSettings {
   WorkspaceSettings {
     enable: Some(true),
+    disable_paths: vec![],
     enable_paths: None,
     config: None,
     certificate_stores: None,
diff --git a/cli/tests/integration/lsp_tests.rs b/cli/tests/integration/lsp_tests.rs
index 64c59ce7d9..6363a08f63 100644
--- a/cli/tests/integration/lsp_tests.rs
+++ b/cli/tests/integration/lsp_tests.rs
@@ -1298,13 +1298,14 @@ fn lsp_inlay_hints_not_enabled() {
 }
 
 #[test]
-fn lsp_workspace_enable_paths() {
+fn lsp_workspace_disable_enable_paths() {
   fn run_test(use_trailing_slash: bool) {
     let context = TestContextBuilder::new().use_temp_cwd().build();
     let temp_dir = context.temp_dir();
     temp_dir.create_dir_all("worker");
     temp_dir.write("worker/shared.ts", "export const a = 1");
     temp_dir.write("worker/other.ts", "import { a } from './shared.ts';\na;");
+    temp_dir.write("worker/node.ts", "Buffer.alloc(1);");
 
     let root_specifier = temp_dir.uri();
 
@@ -1312,6 +1313,7 @@ fn lsp_workspace_enable_paths() {
     client.initialize_with_config(
       |builder| {
         builder
+          .set_disable_paths(vec!["./worker/node.ts".to_string()])
           .set_enable_paths(vec!["./worker".to_string()])
           .set_root_uri(root_specifier.clone())
           .set_workspace_folders(vec![lsp::WorkspaceFolder {
@@ -1329,6 +1331,7 @@ fn lsp_workspace_enable_paths() {
       },
       json!([{
         "enable": false,
+        "disablePaths": ["./worker/node.ts"],
         "enablePaths": ["./worker"],
       }]),
     );
@@ -1395,6 +1398,17 @@ fn lsp_workspace_enable_paths() {
     );
     assert_eq!(res, json!(null));
 
+    let res = client.write_request(
+      "textDocument/hover",
+      json!({
+        "textDocument": {
+          "uri": root_specifier.join("./worker/node.ts").unwrap(),
+        },
+        "position": { "line": 0, "character": 0 }
+      }),
+    );
+    assert_eq!(res, json!(null));
+
     let res = client.write_request(
       "textDocument/hover",
       json!({
diff --git a/test_util/src/lsp.rs b/test_util/src/lsp.rs
index d276e3ed36..2af27e8d44 100644
--- a/test_util/src/lsp.rs
+++ b/test_util/src/lsp.rs
@@ -388,6 +388,12 @@ impl InitializeParamsBuilder {
     self
   }
 
+  pub fn set_disable_paths(&mut self, value: Vec<String>) -> &mut Self {
+    let options = self.initialization_options_mut();
+    options.insert("disablePaths".to_string(), value.into());
+    self
+  }
+
   pub fn set_enable_paths(&mut self, value: Vec<String>) -> &mut Self {
     let options = self.initialization_options_mut();
     options.insert("enablePaths".to_string(), value.into());