diff --git a/cli/dts/lib.deno.unstable.d.ts b/cli/dts/lib.deno.unstable.d.ts
index b83309bc9d..fb5ed461cf 100644
--- a/cli/dts/lib.deno.unstable.d.ts
+++ b/cli/dts/lib.deno.unstable.d.ts
@@ -142,7 +142,7 @@ declare namespace Deno {
    * Opens a dynamic library and registers symbols
    */
   export function dlopen<S extends Record<string, ForeignFunction>>(
-    filename: string,
+    filename: string | URL,
     symbols: S,
   ): DynamicLibrary<S>;
 
diff --git a/ext/ffi/00_ffi.js b/ext/ffi/00_ffi.js
index 3c4112a473..553ea1dd36 100644
--- a/ext/ffi/00_ffi.js
+++ b/ext/ffi/00_ffi.js
@@ -3,6 +3,7 @@
 
 ((window) => {
   const core = window.Deno.core;
+  const __bootstrap = window.__bootstrap;
 
   class DynamicLibrary {
     #rid;
@@ -23,7 +24,9 @@
   }
 
   function dlopen(path, symbols) {
-    return new DynamicLibrary(path, symbols);
+    // URL support is progressively enhanced by util in `runtime/js`.
+    const pathFromURL = __bootstrap.util.pathFromURL ?? ((p) => p);
+    return new DynamicLibrary(pathFromURL(path), symbols);
   }
 
   window.__bootstrap.ffi = { dlopen };